Work

Understanding physiochemical changes in Jurkat cells at different layers during freezing using Raman Spectroscopy

Year: 2016

Type: other

Abstract: Cryopreservation is a process where cells or tissues are preserved by cooling to sub-zero temperatures, where any enzymatic or chemical reaction is stopped due to lack of thermal energy. Previous experiments studied the changes in cells at one specific layer. However, formation of ice on the surface, then into cell during the cooling process will affect image analysis. In this study, the physiochemical changes in Jurkat cells at three different layers were studied based on the distribution of ice, cryoprotectant, and cytochrome C using Raman Spectroscopy.

Source: University of Minnesota Digital Conservancy (University of Minnesota)

Authors: Guanglin Yu, Yan Rou Yap, Allison Hubel

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2464230540

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