Work

Sugars waste, an alternative growth and complete medium for fast growing Rhizobium cells

Year: 2011

Type: article

Abstract: The sugar waste (molasses) was tested for various physiochemical parameters. After examination of various physiochemical parameters, growth and population count ofRhizobium meliloti MTCC-100 was monitored at different concentrations of sugar waste (10, 20, 30 and up to 100%) in terms of optical density (OD) and colony forming unit (C.F.U.). Growth and cell count of Rhizobium were highest in 10% sugar waste concentration. Growth pattern of the bacteria was observed at 10% sugar waste along with different synthetic media (tryptone yeast extract medium, rhizobium minimal medium and yeast extract medium). Growth of bacteria in 10% sugar waste was found to be superior to standard media (TY, RMM and YEM) used for Rhizobium. The important environmental parameters like pH and temperature were optimized for 10% sugar waste as growth medium. A pH of 7.0 and temperature of 28°C were found to be the most suitable for the fast growing R. meliloti MTCC-100. Key words: Rhizobium meliloti, sugar waste, synthetic media, colony forming unit.

Source: African Journal of Microbiology Research

DOI: 10.5897/ajmr11.408

Authors: A, Singh G, P Bhatt R, Pant S, Naglot A, Singh L

Institutions: Dolphin (PG) Institute of Bio Medical and Natural Science, Hemwati Nandan Bahuguna Garhwal University, Defence Research and Development Organisation, Defence Research Laboratory

Language: English

Topic: Agriculture, Plant Science, Crop Management

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Responsible consumption and production, Zero hunger

Open Access status: diamond

OpenAlex ID: W2466000596