Work

Capability of in vitro digestibility methods to predict in vivo digestibility of vegetal and animal proteins

Year: 2026

Type: article

Abstract: The purpose of this work was to establish predictive equations for the digestibility of proteins of animal and vegetal origin by correlating in vitro and in vivo methods. Proteins sources for animal and vegetable were used. To calculate in vitro digestibility, we used pH values obtained 10 min after a solution of enzymes was added to a protein solution (pH-drop method). We also used the pH-static method, which measures the volume of additional NaOH that is necessary to maintain a pH of 8.0 after the addition of an enzymatic solution. In vivo digestibility was measured in newly weaned male rats that were fed a diet of AIN-93G for growth with a modified protein content of 9.5% for 14 days. The equations developed using the pH-drop method allowed us to predict in vivo digestibility amounts that were more closely correlated with real in vivo digestibility than those obtained with equations using the pH-static method. In vitro techniques are less expensive, require less manpower and physical space, and use a smaller quantity of protein.

Source: Archivos Latinoamericanos de Nutrición

DOI: 10.37527/2016.66.1.001

Authors: Fabrícia Queiroz Mendes, Maria Goreti De Almeida Oliveira, Neuza Maria Brunoro Costa, Christiano Vieira Pires, Flávia Regina Passos

Institutions: Universidade Federal de Viçosa, Universidade Federal do Espírito Santo, Federal University of São João del-Rei

Language: English

Topic: Animal Nutrition and Physiology

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $300