Work

Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests

Year: 2016

Type: article

Abstract: Antibodies have traditionally served as the affinity reagents of choice in point-of-care diagnostic biosensors. However, this class of proteins is not ideally suited for this use, being poorly characterized and prone to thermal denaturation. Here, we present an activity-based assessment of an alternative engineered binding protein in a cellulose-based assay.

Source: Molecular Systems Design & Engineering

DOI: 10.1039/c6me00032k

Authors: E. A. Miller, M. W. Traxlmayr, J. Shen, H. D. Sikes

Institution: Massachusetts Institute of Technology

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Interdepartmental Biotechnology Training Program