Work

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Year: 2016

Type: article

Abstract: A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, the macrophages can be harvested and seeded into culture wells at required cell densities for carrying out experiments. The use of defined numbers of macrophages rather than defined numbers of monocytes to initiate macrophage cultures for experiments yields macrophage cultures in which the desired cell density can be more consistently attained. Use of cryopreserved monocytes reduces dependency on donor availability and produces more homogeneous macrophage cultures.

Source: Journal of Visualized Experiments

DOI: 10.3791/54244

Authors: Xueting Jin, Howard S. Kruth

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Immune cells in cancer

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2468493734

FWCI: 2.221

Cites: 23

Cited by: 66

Related to: 10