Spectrophotometric determination of artemisinin and dihydroartemisinin
Year: 2008
Type: article
Abstract: A simple and rapid spectrophotometric method for the determination of artemisinin (ART) and dihydroartemisinin (DHA) is described. The method is based on the reaction of hydrogen peroxide (H 2 O 2 ), generated by the cleavage of endoperoxide linkage of ART/DHA and its reaction with potassium iodide to liberate iodine. The liberated iodine bleaches the red coloured safranin O to colourless species and is measured at 521 nm. Beer's law is obeyed in the range of 16-112 μg mL -1 for both ART and DHA. The molar absorptivity, Sandell's sensitivity, detection limit and quantitation limit for ART were found to be 0.3401x10 4 Lmol -1 cm -1 , 1.43 x 10 -2 μg cm -2 , 0.0685 μg mL -1 and 0.2075 μg mL -1 respectively; while that for DHA were found to be 0.2891 x 10 4 L mol -1 cm -1 , 1.33 x 10 -2 μg cm -2 , 0.0491 μg mL -1 and 0.1488 μg mL -1 respectively. The optimum reaction conditions and other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision.
Authors: T.V. Sreevidya, B. Narayana
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2468820773