QRT-PCR confirms two separate CenpA domains in the neocentromeric cell line BBB
Year: 2011
Type: other
Abstract: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed on equal amounts of total DNA obtained from centromere protein (CENP)-A chromatin immunoprecipitation (ChIP) DNA and Input DNA from BBB cell line. The thirty-four PCR primer pairs used (shown as black lines in the x-axis) amplified fragments from 150 to 250 base pairs contained within the 350 kb neocentromere region (see Figure 2). Each primer pair was assayed in at least three independent CENP-A ChIP experiments. The qRT-PCR results for each primer pair were expressed on the y-axis as the fold enhancement between the CENP-A ChIP DNA and input DNA (= 1.93) normalized to the value obtained for the positive control alpha satellite DNA primer pair (far right). The shaded region indicates the area determined to be the CENP-A domain in Figure 2. The 34 qRT-PCR primer pairs and the 133 PCR products from this region on the PCR microarray (Figure 2) are shown. qRT-PCR primers that amplified products wholly contained within a PCR microarray fragment are indicated by numbers in parentheses; the rest are labeled alphabetically. Only qRT-PCR fragments shown in Table 1 are indicated; information for all other primers can be found in the Additional data file 3. CENP-A domains derived from the PCR microarray data are indicated. Genome coordinates correspond to the region of chr13 from the Human Genome Browser at UCSC (hg17) [50].Copyright information:Taken from "Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres"http://genomebiology.com/2007/8/7/R148Genome Biology 2007;8(7):R148-R148.Published online 25 Jul 2007PMCID:PMC2323242.
Source: Figshare
Authors: Alonso, Alicia, Fritz, Björn, Hasson, Dan, Abrusan, György, Cheung, Fanny, Yoda, Kinya, Radlwimmer, Bernhard, Ladurner, Andreas G, Warburton, Peter E
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W6977302362