Non-Invasive Differentiation of Benign Renal Tumors from Clear Cell Renal Cell Carcinomas Using Clinically Translatable Hyperpolarized 13C Pyruvate Magnetic Resonance
Year: 2016
Type: article
Abstract: Incidental detection of localized renal tumors at imaging is increasing. Conventional imaging cannot reliably differentiate the 20% of these tumors that are benign from malignant renal cell carcinomas (RCCs), leading to unnecessary surgical resection and resulting morbidity. Here, we investigated hyperpolarized 13C pyruvate metabolism in live patient-derived renal tumor tissue slices using a novel magnetic resonance-compatible bioreactor platform. We show, for the first time, that clear cell RCCs (ccRCCs), which constitute 70%–80% of all RCCs, exhibit increased lactate production and rapid lactate efflux when compared with benign renal tumors. This difference is because of increased lactate dehydrogenase A and monocarboxylate transporter 4 expression in ccRCCs. Thus, RCCs can be differentiated from benign renal tumors by assessing this distinctive metabolic phenotype using clinically translatable hyperpolarized 13C pyruvate magnetic resonance.
Source: Tomography
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,896
Funders: U.S. Department of Defense, National Institutes of Health, University of California, San Francisco, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Biomedical Imaging and Bioengineering, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, Medical Research and Materiel Command
Awards: G1059885655, G1600419044, G1802360313, Ascorbate-based biomarkers for predicting radiation response in prostate cancer, G3994034673, G41694728, G5613049967, G605929298, Novel Hyperpolarized MR Markers of Advanced Prostate Cancer Therapy, Human Tissue Culture Bioreactor and Hyperpolarized MR for Biomarker Discovery, G6699015319, G9000003249, G9000048842, G9000124270, G9000132684, G9000181940, Cancer Center Support Grant, Hyperpolarized MRI Technology Resource Center
OpenAlex ID: W2471552358