Cellular mechanisms involved in the induction and maintenance of long-term potentiation (LTP) in the spinal dorsal horn
Year: 2010
Type: dissertation
Abstract: The cellular processes leading to spinal long-term potentiation (LTP) are regarded as underlying mechanisms of sensitization in the dorsal horn. In this study, spinal LTP was induced by high-frequency stimulation (HFS) conditioning of the sciatic nerve. Electrophysiological extracellular recordings from nociceptive single\ndorsal horn neurons were used in combination with quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) to examine the mechanisms for induction and maintenance of spinal LTP. \nSpinal administration of the N-methyl-D-aspartate-2B (NMDA-2B) receptor antagonist Ro 25-6981 showed an antinociceptive effect on spinal dorsal horn neuronal activity and clearly attenuated the magnitude of spinal LTP. Moreover, induction of LTP after HFS\nconditioning was not observed following pre-treatment of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor AIP. A transient increase in the expression of the gene for the transcription factor Zif268 was observed in the spinal cord 120 minutes after HFS conditioning. Further, the expression of the genes for interleukin-1β (IL-1β), glial cell linederived neurotrophic factor (GDNF) and inducible nitric oxide synthase (iNOS) increased significantly in the ipsilateral dorsal horn 360 minutes after HFS conditioning. \nThese data demonstrate that activation of the spinal NMDA-2B receptor and the intracellular CaMKII enzyme may be important for the induction of spinal LTP. Moreover our results indicate that increased gene expression of Zif268, IL-1β, GDNF and iNOS following HFS might be associated with the maintenance of spinal LTP.
Author: Linda M. Pedersen
Language: English
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2473038494