Work

At the Interface of Chemical and Biological Synthesis: An Expanded Genetic Code

Year: 2016

Type: preprint

Abstract: The ability to site-specifically incorporate noncanonical amino acids (ncAAs) with novel structures into proteins in living cells affords a powerful tool to investigate and manipulate protein structure and function. More than 200 ncAAs with diverse biological, chemical, and physical properties have been genetically encoded in response to nonsense or frameshift codons in both prokaryotic and eukaryotic organisms with high fidelity and efficiency. In this review, recent advances in the technology and its application to problems in protein biochemistry, cellular biology, and medicine are highlighted.

Source: Cold Spring Harbor Perspectives in Biology

DOI: 10.1101/cshperspect.a023945

Authors: Han Xiao, Peter G. Schultz

Institutions: Scripps Research Institute, California Institute for Biomedical Research

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: U.S. Department of Energy

Award: BIOPOLYMERS CONTAINING UNNATURAL BUILDING BLOCKS