Work

Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis

Year: 2016

Type: article

Abstract: We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.00857-16

Authors: Xiaohong Zeng, Hui Li, Rongrong Zheng, Natalia Kurepina, Barry N. Kreiswirth, Xilin Zhao, Ye Xu, Qingge Li

Institutions: Xiamen University, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Henan Provincial Center for Disease Control and Prevention, Rutgers, The State University of New Jersey, Rutgers New Jersey Medical School

Language: English

Topic: Tuberculosis Research and Epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Natural Science Foundation of China

Award: G8798895026

OpenAlex ID: W2474043068

FWCI: 1.128

Cites: 20

Cited by: 17

Related to: 10