[Bifidobacterium cell wall proteins induced beta-defensin 2 mRNA expression in human intestinal epithelial cells].
Year: 2003
Type: article
Abstract: OBJECTIVE: To examine Bifidobacterium mediated induction of human defensin 2 mRNA expression in human intestinal epithelial cells and define the bioactive components of Bifidobacterium. METHODS: Bifidobacterium was cultured in MRS medium in anaerobic incubator and its cell wall was isolated by sonication and centrifugation. The cell wall proteins of Bifidobacteria were extracted with 2% SDS. The level of hBD-2 mRNA in HT-29 cells was detected by reverse-transcription PCR and Northern blot analysis. RESULTS: There was no detectable signal of hBD-2 mRNA in the unstimulated HT-29 cells. However, the heat-killed Bifidobacteria, cell wall of Bifidobacteria, and cell wall proteins remarkably induced hBD-2 mRNA expression in HT-29. CONCLUSION: Bifidobacterium or its cell wall proteins can induce hBD-2 gene expression in human intestinal epithelial cells, which may play a role in the mechanisms of innate defense against pathogens in the intestine.
Source: PubMed
Language: Chinese
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W646662492