Year: 2014
Type: article
Abstract: Currently available microbial genomes are of limited phylogenetic breadth due to ourhistorical inability to cultivate most microorganisms in the laboratory. The firstphase of the Microbial Dark Matter project used single-cell genomics to sequence 201single cells from uncultivated lineages, and was able to resolve new superphyla andreveal novel metabolic features in bacteria and archaea. However, many fundamentalquestions about the evolution and function of microbes remain unanswered, and manycandidate phyla remain uncharacterized. Phase II of the Microbial Dark Matter projectwill target candidate phyla with no sequenced representatives at a variety of new sites usinga combination of single-cell sequencing and shotgun metagenomics approaches.
Authors: Jessica Jarett, Peter F. Dunfield, Sari Peura, Paul W. J. J. van der Wielen, Brian P. Hedlund, Mostafa S. Elshahed, Konstantinos Ar. Kormas, Andreas Teske Stott, Nils-Kåre Birkeland, Chuanlun Zhang, Karin Rengefors, Stephen R. Lindemann, Nikolai V. Ravin, John R. Spear, Steven Hallam, Sean A. Crowe, Jillian Steele, Danielle Goudeau, Rex R. Malmstrom, Nikos C. Kyrpides, Ramûnas Stepanauskas, Tanja Woyke
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2476796882