Relationship of DNA repair processes to mutagenesis and carcinogenesis in mammalian cells. Progress report, November 1, 1978-October 31, 1979. [EMS; cultured mouse embryo cells (C3H/10T 1/2)]
Year: 1979
Type: report
Abstract: The objective of this research is to determine the role of DNA repair in mutagenesis and carcinogenesis in mammlian cells. Repair capabilities will be varied by: (1) using wild-type and repair-deficient cell lines; (2) imposing various recovery periods following mutagenic treatment; (3) exposing cells to inhibitors of DNA repair following mutagenic treatment; and (4) varying the mutagenic treatment to cause DNA lesions repairable by different pathways. Reconstruction experiments were conducted to (1) investigate conditions for measuring transformation frequency using colony formation, and (2) determine the influence of ouabain-sensitive cells on the expression of ouabain-resistance. Ouabain-resistant cells were isolated from EMS-treated cultures and were shown to be resistant to the drug with regard to survival and the activity of the Na/sup +/-K/sup +/-ATPase. The effect of holding EMS-treated cells under various conditions which do not allow DNA synthesis was determined with respect to survival and the incidence of transformation and mutation. When confluent cells were held for 4 hrs following EMS treatment, both survival and the frequency of transformation increased. When the cells were incubated in 0.1% serum for 6 hrs following EMS treatment, no change in either the transformation or mutation frequency was observed as compared to cells incubated in 10% serum. Further experiments are in progress to determine the effect of recovery periods on transformation and mutation frequencies.
DOI: 10.2172/5756787
Author: H.H. Evans
Institution: Case Western Reserve University
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: U.S. Department of Energy
OpenAlex ID: W2478477737