Work

Polydiacetylenyl β-cyclodextrin based smart vesicles for colorimetric assay of arginine and lysine

Year: 2016

Type: article

Abstract: Selective visualization of arginine and lysine has been explored among 20 amino acids using the hybrid conjugate of β-cyclodextrin (β-CD) and polydiacetylene (PDA). The mono pentacosa-10,12-diynyl aminomethyl group was successfully coupled to either the primary or the secondary face of β-CD, where mono-6-amino-6-deoxy-β-CD or mono-3-amino-3-deoxy-β-CD reacted with the N-hydroxysuccinimide ester of 10,12-pentacosadiynoic acid. In this combinatorial system, the cylindrical β-cyclodextrin functions as a channel for the introduction of the cationic amino acids to the artificial membrane. The membrane perturbation and aggregation by the target amino acids could be exclusively visualized as a blue to red color change based on the responsive polydiacetylene domain. These interesting findings demonstrated that the developed β-CD conjugated PDA system may offer a new method of cell-penetrating mechanism, a promising vector system, as well as impact the production industry of arginine or lysine.

Source: Scientific Reports

DOI: 10.1038/srep31115

Authors: Eunae Cho, Hwanhee Kim, Youngjin Choi, Seung R. Paik, Seunho Jung

Institutions: Konkuk University, Hoseo University, Seoul National University

Language: English

Topic: Polydiacetylene-based materials and applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: National Research Foundation, Konkuk University, Ministry of Science, ICT and Future Planning, National Research Foundation of Korea