Year: 2013
Type: article
Abstract: Background and Aims: During the last years a new Foreskin cell line strain (RFSC) has been isolated, characterized and stored in a representative cell bank stock in liquid nitrogen as a part of HCB (Human Cell Bank) development program in Razi institute. Materials and Methods:The cell population was serially passaged for mass production purpose based on WHO schedule .The controls on pool samples were carried out upon our sampling method and international recommendations.Results: The data concerning parameters as well as the result of compulsory controls on Kario logy oncogenicity, cross contaminations, species specificity, no oncogenicity, and mycoplasma free of a human cell line that contained heteroploid chromosomal characterizations are performed.Conclusion: Kariological examination found an obvious discrepancy in the frequency of aberrations due to high passage level that produced a heteroploid continuous cell line.Virus susceptibility shows RFSC-1 more sensitive to RS#12 Mumps virus strain.This cell line is completely sterile and has no any cross contamination to other pathogen and can be used for production and control of Mumps vaccine.
Source: Iranian Journal of Virology
DOI: 10.21859/isv.7.4.7
Authors: N Mahmudi-Gharoeie, A Mohammadi, B Saffar, F Esna-Ashari, A Foroghi, B Alirezaee, R Ghorbani, ZA Sedigh
Language: English
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W2352258190