Work

Enzymatic Activity Assays in Yeast Cell Extracts

Year: 2014

Type: article

Abstract: ) (commonly known as baker's yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell extracts from yeast to investigate their enzymatic activities. This protocol is a quick and efficient way to make yeast cell extracts without using commercial kits.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.1312

Authors: Melike Çağlayan, Samuel Wilson

Institutions: National Institutes of Health, National Institute of Environmental Health Sciences

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Environmental Health Sciences

Awards: Biology of Mammalian Base Excision Repair and HIV-1 Replication, Mechanisms of Nucleic Acid Enzymes - Pol Beta and Mammalian Base Repair Enzymes, Biology of Mammalian Base Excision Repair, Mechanisms of Nucleic Acid Enzymes - Pol Beta, HIV-1 Reverse Transcriptase and Mammalian Base Repair Enzymes