Work

Label-Free Detection of Single Living Bacteria via Electrochemical Collision Event

Year: 2016

Type: article

Abstract: We detected single living bacterial cells on ultramicroelectrode (UME) using a single-particle collision method and optical microscopic methods. The number of collision events involving the bacterial cells indicated in current-time (i-t) curves corresponds to the number of bacterial cells (i.e., Escherichia coli) on the UME surface, as observed visually. Simulations were performed to determine the theoretical current response (75 pA) and frequency (0.47 pM(-1) s(-1)) of single Escherichia coli collisions. The experimental current response (83 pA) and frequency (0.26 pM(-1) s(-1)) were on the same order of magnitude as the theoretical values. This single-particle collision approach facilitates detecting living bacteria and determining their concentration in solution and could be widely applied to studying other bacteria and biomolecules.

Source: Scientific Reports

DOI: 10.1038/srep30022

Authors: Ji Young Lee, Byung-Kwon Kim, Mijeong Kang, Jun Hui Park

Institutions: Korea Advanced Institute of Science and Technology, Sookmyung Women's University, University of Maryland, College Park, Jeonbuk National University

Language: English

Topic: Electrochemical Analysis and Applications

Subfield: Electrochemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,690