Work

Sir protein–independent repair of dicentric chromosomes inSaccharomyces cerevisiae

Year: 2016

Type: article

Abstract: Sir2 protein has been reported to be recruited to dicentric chromosomes under tension, and such chromosomes are reported to be especially vulnerable to breakage in sir2Δ mutants. We found that the loss of viability in such mutants was an indirect effect of the repression of nonhomologous end joining in Sir(-) mutants and that the apparent recruitment of Sir2 protein to chromosomes under tension was likely due to methodological weakness in early chromatin immunoprecipitation studies.

Source: Molecular Biology of the Cell

DOI: 10.1091/mbc.e16-02-0102

Authors: David F. McCleary, David Lee Steakley, Jasper Rine

Institutions: National Institutes of Health, University of California, Berkeley

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences