Year: 2015
Type: dissertation
Abstract: Myogenesis is a complex process that is markedly affected by environmental factors.In particular, the phenotype of myotomal muscle in zebrafish (Danio rerio) is known to undergo modifications in response to temperature changes.Despite of their demonstrated importance in numerous biological processes, the role of microRNAs (miRNAs) in regulation of muscle growth and adaptation to environmental changes is still poorly understood in fish.To investigate the thermal plasticity of miRNA expression and their role in myogenesis and thermal acclimation, zebrafish were reared from fertilized eggs to adulthood at three different temperatures (24.3 ± 0.8 ºC, 28.3 ± 0.3 ºC, and 33.4 ± 0.4 ºC).Expression profile analysis revealed differentially expressed miRNAs and mRNAs, which were affected by rearing temperature.Many differentially expressed mRNAs were involved in muscle structure development and contraction, detection of cell damage, energy metabolism and response to oxidative stress.Most miRNAs displaying significant changes in mature transcript levels were not previously reported as miRNAs related to temperature response and muscle development.For example, miR-218a, miR-200a, miR-100, miR-99, miR-26b, miR-101b and let-7e were shown to be the most significantly differentially expressed miRNAs associated with longterm cold and warm acclimation.Analysis of their putative interaction with differentially expressed mRNAs suggested that these miRNAs may have a role in myogenesis and muscle recovery.This study contributed to our limited understanding of the role of miRNAs in acclimation and muscle development in fish.
Source: BIBSYS Brage (BIBSYS (Norway))
Author: Anastasia Pesiakova
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Award: G5185714120
OpenAlex ID: W2501081287