Work

Tetraspanin 8 mediates AEG ‐1‐induced invasion and metastasis in hepatocellular carcinoma cells

Year: 2016

Type: article

Abstract: Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migration without affecting proliferation. TSPAN8 knockdown profoundly abrogated AEG-1-induced primary tumor and intrahepatic metastasis in an orthopic xenograft model in athymic nude mice. Coculture of TSPAN8 knockdown cells with human umbilical vein endothelial cells (HUVEC) markedly inhibited HUVEC tube formation indicating that inhibition of angiogenesis might cause reduction in primary tumor size. TSPAN8 inhibition might be a potential therapeutic strategy for metastatic HCC.

Source: FEBS Letters

DOI: 10.1002/1873-3468.12268

Authors: Maaged A. Akiel, Prasanna K. Santhekadur, Rachel G. Mendoza, Ayesha Siddiq, Paul B. Fisher, Devanand Sarkar

Institutions: Virginia Commonwealth University, VCU Massey Comprehensive Cancer Center

Language: English

Topic: Cancer Mechanisms and Therapy

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Samuel Waxman Cancer Research Foundation, National Institutes of Health, National Cancer Institute, National Institute of Neurological Disorders and Stroke