The Expression of Cell Surface Heparan Sulfate Proteoglycans and Their Roles in Turkey Skeletal Muscle Formation
Year: 2003
Type: article
Abstract: Skeletal muscle myogenesis is a series of highly organized processes including cell migration, adhesion, proliferation, and differentiation that are precisely regulated by the extrinsic environment of muscle cells.Fibroblast growth factor 2 (FGF2) is one of the key growth factors involved in the regulation of skeletal muscle myogenesis.Since FGF2 is a potent stimulator of skeletal muscle cell proliferation but an intense inhibitor of cell differentiation, changes in FGF2 signaling to muscle cells will influence cell behavior and result in differences in cell proliferation and differentiation.As the cell surface heparan sulfate proteoglycans (HSPG), syndecans and glypicans, are the lowaffinity receptors of FGF2 and function to regulate the binding of FGF2 to the highaffinity fibroblast growth factor receptors (FGFR) and affect the activity of FGF2, differences in the expression of these molecules may cause alterations in cell responsiveness to FGF2 stimulation, which can lead to changes in skeletal muscle development and growth.However, the precise functional differences of syndecans and glypicans in FGF2 signaling are unknown to date.Our hypothesis is that syndecans and glypicans may play different roles in regulating the FGF2-FGFR interaction, and the relative expression of these molecules is critical for determining the cell status in proliferation and differentiation.To address how changes in the expression of cell surface HSPG syndecan-1 and glypican-1 are related to muscle growth properties, and iii how the differences in the expression of syndecan-1 and glypican are related to FGF2 and FGFR 1 expression, a turkey line (F) selected for increased body weight at 16-wk posthatch and its control line (RBC2) which was unselected for any trait were used as the animal model.The pectoralis major muscle tissue isolated from individuals of the F and RBC2 line at different embryonic and posthatch developmental ages as well as myogenic satellite cells isolated from these two lines of turkeys were used to investigate the expression of syndecan-1, glypican, FGF2, and FGFR 1 during in vivo and in vitro myogenesis.In addition to line and developmental differences, the sex differences in syndecan-1, glypican, FGF2, and FGFR 1 expression were also measured.The results showed that in the F line satellite cells the increase in syndecan-1 expression was correlated with the increase in FGF2 expression and cell proliferation as compared to the RBC2 line cells.The expression of syndecan-1 and glypican expression was enhanced in the F line p. major muscle compared to the RBC2 line samples during both embryonic and posthatch development.The results from this study provide novel information about the expression of syndecan-1 and glypican as it relates to FGF2 signaling and skeletal muscle formation.
Author: Xiaosong Liu
Institution: The Ohio State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2508024520