Work

A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery

Year: 2016

Type: article

Abstract: BACKGROUND: Fluorescence-guided surgery (FGS) of tumors is an area of intense development. Peritoneally-disseminated cancer, however, represents a difficult surgical challenge. MATERIALS AND METHODS: To help meet this challenge, EL4 mouse T-cell lymphoma cells expressing red fluorescent protein (RFP) were injected intraperitoneally in nude mice. RESULTS: Two weeks after injection of EL4-RFP cells, established peritoneally-disseminated tumors were observed. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). FGS enabled detection of very small peritoneal disseminated tumors and completely resected them in contrast to bright-light which only partially detected the tumors. CONCLUSION: The present report indicates the feasibility of FGS of peritoneally-disseminated cancer.

Source: Anticancer Research

DOI: 10.21873/anticanres.10993