Androgen receptor differentially regulates the proliferation of prostatic epithelial cellsin vitroandin vivo
Year: 2016
Type: article
Abstract: // Shu Yang 1, * , Ming Jiang 2, 3, * , Magdalena M. Grabowska 4 , Jiahe Li 1 , Zachary M. Connelly 1 , Jianghong Zhang 4 , Simon W. Hayward 5 , Justin M. Cates 6 , Guichun Han 7 , Xiuping Yu 1 1 Department of Biochemistry and Molecular Biology, LSU Health Sciences Center, Shreveport, LA, USA 2 Laboratory of Nuclear Receptors and Cancer Research, Center for Basic Medical Research, Nantong University School of Medicine, Nantong, Jiangsu, China 3 Institute of Medicine and Public Health, Division of Epidemiology, Department of Medicine, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, USA 4 Department of Urologic Surgery, Vanderbilt University Medical Center, Nashville, TN, USA 5 Department of Surgery, NorthShore University HealthSystem Research Institute, Evanston, IL, USA 6 Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA 7 Women’s Health Division, Michael E. DeBakey Institute, and Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA * These authors have contributed equally to this work Correspondence to: Xiuping Yu, email: [email protected] Keywords: prostate, androgen receptor Received: April 23, 2016 Accepted: August 24, 2016 Published: September 7, 2016 ABSTRACT Androgens regulate the proliferation and differentiation of prostatic epithelial cells, including prostate cancer (PCa) cells in a context-dependent manner. Androgens and androgen receptor (AR) do not invariably promote cell proliferation; in the normal adult, endogenous stromal and epithelial AR activation maintains differentiation and inhibits organ growth. In the current study, we report that activation of AR differentially regulates the proliferation of human prostate epithelial progenitor cells, NHPrE1, in vitro and in vivo . Inducing AR signaling in NHPrE1 cells suppressed cell proliferation in vitro , concomitant with a reduction in MYC expression. However, ectopic expression of AR in vivo stimulated cell proliferation and induced development of invasive PCa in tissue recombinants consisting of NHPrE1/AR cells and rat urogenital mesenchymal (UGM) cells, engrafted under renal capsule of adult male athymic mice. Expression of MYC increased in the NHPrE1/AR recombinant tissues, in contrast to the reduction seen in vitro . The inhibitory effect of AR signaling on cell proliferation in vitro were reduced by co-culturing NHPrE1/AR epithelial cells with prostatic stromal cells. In conclusion, these studies revealed that AR signaling differentially regulates proliferation of human prostatic epithelia cells in vitro and in vivo through mechanisms involving stromal/epithelial interactions.
Source: Oncotarget
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2512367653