Work

Go-Rex: A Novel in Vitro System for the Assessment of CAR T Cell Function

Year: 2016

Type: article

Abstract: The preclinical development of chimeric antigen receptor (CAR) T cell therapy has been hindered by the inadequacy of current in vitro methods to predict T cell performance (i.e. migration, proliferation and anti-tumor activity), which do not recapitulate in vivo conditions. For example, most T cell functional assays are performed over a relatively short timeframe (6-18 hrs), and platforms to assess T cell migration and prolonged T cell - tumor cell interactions are very restricted. To overcome these deficits, we developed the Go-Rex – an in vitro, compartmentalized culture system that allows long-term assessment of multiple biological parameters simultaneously.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2015.11.970