Escherichia coli pathotypes from Ecuador: association with diarrhea and antibiotic resistance
Year: 2016
Type: dissertation
Study design: Observational Study
Abstract: Diarrheagenic Escherichia coli (DEC) is an important cause of diarrhea in the developing world and the detection of these bacteria and their antibiotic resistance profiles is necessary for effective therapy. In this study, we conducted a microbiological survey of DEC in 233 stool samples, collected during a case control study in a hospital and health center in a low income neighborhood of Quito, Ecuador from April to September 2014. We used 8 sets of PCR primers to detect distinct DEC pathotypes. The overall prevalence of DEC was 30.5% in cases and 20.2% in controls (OR 1.76 CI 95% 0.96-3.20, p=0.06), Diffusely adherent E.coli (DAEC) was the most frequently detected pathotype in cases and controls (15.3% vs. 6.1% respectively) and was the only pathotype with a statistically significant association with diarrhea (OR 2.78, CI 95% 1.11-6.96, p=0.03). To our knowledge this is the first study investigating this pathotype in Ecuador. Additionally, pathotypes isolated from cases exhibited significantly higher levels of antimicrobial resistance to specific antibiotics, as well as higher levels of multidrug resistance, than isolates obtained from controls.
Authors: Montero Toro, Lorena Patricia
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2515210128