Oxygen Consumption, Muscle Fibrosis, and Oxidative Stress in the mdx mouse: Influence of Treadmill Running
Year: 2014
Type: article
Abstract: Dystrophin protein absence in the mdx mouse leads to myofiber necrosis and oxidative stress, and exercise accelerates a cascade of events resulting in muscle damage.Examining the influence of exercise on oxygen consumption, oxidative stress and muscle fibrosis will help to enhance the understanding of these pathological mechanisms.PURPOSE: To test the effects of a four-week treadmill exercise program on oxygen consumption and oxidative stress, and muscle fibrosis in the mdx mouse in attempt to improve endpoint measures of the disease that can be used to monitor treatment effectiveness.METHODS: Seven mdx (EX) mice aged four weeks were subjected to four weeks of twice-weekly treadmill exercise for 30 minutes at 12 m/min on a rodent treadmill (Columbus Instruments).Nine mdx (SED) mice and five wild type C57-BL/10 mice (WT) served as non-exercise sedentary controls.All mice underwent two graded V02 max tests; at three days prior to four weeks of age, and again at three days after eight weeks of age.O2 consumption and CO2 production were measured continuously during max testing.Total time to exhaustion (TTE) on the treadmill was also measured.Fibrotic skeletal muscle damage in isolated quadriceps was determined by the degree of hydroxyproline deposition.Oxidative stress was measured in quadriceps, cardiac, and abdominal muscle by measuring glutathione oxidation.Muscle cryosections of approximately 8 μm were stained for intracellular immunoglobulin G (IgG) and Collagen Vita 2004 ...........
Author: Kevin E. Schill
Institution: The Ohio State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2515676731