Work

Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

Year: 2016

Type: article

Abstract: Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

Source: Molecular Systems Design & Engineering

DOI: 10.1039/c6me00073h

Authors: B. E. Tam, K. Sung, H. D. Sikes

Institution: Massachusetts Institute of Technology

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green