Work

Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis

Year: 2016

Type: article

Abstract: Column-switching high performance liquid chromatography (HPLC) is extensively used for the critical analysis of radiolabeled ligands and their metabolites in plasma. However, the lack of streamlined apparatus and consequently varying protocols remain as a challenge among positron emission tomography laboratories. We report here the prototype apparatus and implementation of a fully automated and simplified column-switching procedure to allow for the easy and automated determination of radioligands and their metabolites in up to 5 mL of plasma. The system has been used with conventional UV and coincidence radiation detectors, as well as with a single quadrupole mass spectrometer.

Source: Pharmaceuticals

DOI: 10.3390/ph9030051

Authors: Neil Vasdev, Thomas Collier

Institutions: Harvard University, Gordon Center for Medical Imaging, Massachusetts General Hospital

Language: English

Topic: Analytical Chemistry and Chromatography

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,499

Funder: Massachusetts General Hospital