Work

A Mutasynthetic Library of Platensimycin and Platencin Analogues

Year: 2016

Type: article

Abstract: Inactivation of ptmB1, ptmB2, ptmT2, or ptmC in Streptomyces platensis SB12029, a platensimycin (PTM) and platencin (PTN) overproducer, revealed that PTM and PTN biosynthesis features two distinct moieties that are individually constructed and convergently coupled to afford PTM and PTN. A focused library of PTM and PTN analogues was generated by mutasynthesis in the ΔptmB1 mutant S. platensis SB12032. Of the 34 aryl variants tested, 18 were incorporated with high titers.

Source: Organic Letters

DOI: 10.1021/acs.orglett.6b02248

Authors: Liao-Bin Dong, Jeffrey D. Rudolf, Ben Shen

Institution: Scripps Research Institute

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Arnold and Mabel Beckman Foundation, National Institute of General Medical Sciences

Awards: G4239711504, Discovery and biosynthesis of diterpenoid natural products in actinomycetals