Uso del factor de selectividad en el diseño conceptual de sistemas para el cultivo de células
Year: 2015
Type: article
Abstract: Mammalian cell culture systems (CCS) are key factors in monoclonal antibody (MAb) production feasibility.Although different processes have been developed for improving cultivation performance, conceptual design is rarely used for selecting the most appropriate cell cultivation strategy.Currently the most common cultivation systems are stirred-tanks, used in fed-batch or single-stage continuous-perfusion operation systems.In present work a structured kinetic model is used to analyze the selectivity of different cell culture systems for MAb production.Ammonia, lactate and cell viability were analyzed as unwanted products.Resulting selectivity equations highlighted different mechanisms affecting MAb yield; all of them being associated to cell growth, cell death, cellular maintenance, glutamine catabolism and glutamine spontaneous decomposition.Results of applying this conceptual analysis to a hybridoma cell model reported in the literature demonstrated that cell death is the main cause of yield loss during a batch cultivation process.Additionally, results suggested that MAb production is favored by double-stage CCS having distributed feeding (a different type of feeding profiles and/or feeding with different compositions).These CCS could be operated in batch, fed-batch or double-stage continuous systems; the latter being the most promising one.Although theoretical results reported here could not be generalized, the applied method may help researchers improving the effectiveness of mammalian cell cultures.At the same time, these results reveal an interesting area of knowledge that should be tested experimentally by physical or computer simulation in mammalian cell culture systems.
Source: ORBi (University of Liège)
Language: Spanish
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2518161119