Synthesis of fluorophore encapsulated silica nanoparticles for the evaluation of the biological fate and toxicity of food relevant nanoparticles
Year: 2014
Type: article
Abstract: This dissertation focuses on three interconnected projects: the characterization of commercial food-relevant nanoparticles during a simulated digestion, and their internalization and toxicity to intestinal epithelial cells; the study of nucleation and growth of quantum dots in a microwave synthesis; and the encapsulation of fluorophores within silica shells for in vitro and in vivo studies to elucidate their fate after ingestion.We show that commercially available TiO 2 , SiO 2 , and ZnO nanoparticles are all internalized by C2BBe1 intestinal epithelial cells, but do not appear to be toxic, even after long term repeat-exposures.When particles were exposed to a simulated digestion protocol mimicking the stomach and intestinal environment, TiO 2 particles did show lung, c) liver, d) brain, and e) spleen.Sections were stained with DAPI (cell nuclei; blue) and E-cadherin (junctions between epithelial cells; red).Rhodamine 6G/silica particles are green.......................
Author: Andrew Zane
Institution: The Ohio State University
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2519404625