Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes
Year: 2016
Type: article
Abstract: There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment. The myosin V globular tail domain (MyoV-GTD) interacts directly with an evolutionarily conserved Spir sequence motif. We determined crystal structures of MyoVa-GTD bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form. The ternary complex architecture explains how Rab11 vesicles support coordinated F-actin nucleation and myosin force generation for vesicle transport and tethering. New insights are also provided into how myosin activation can be coupled with the generation of actin tracks. Since MyoV binds several Rab GTPases, synchronized nucleator and motor targeting could provide a common mechanism to control force generation and motility in different cellular processes.
Source: eLife
DOI: 10.7554/elife.17523
Authors: Olena Pylypenko, Tobias Welz, Janine Tittel, Martin Kollmar, Florian Chardon, Gilles Malherbe, Sabine Weiss, Carina Ida Luise Michel, Annette Samol-Wolf, Andreas Till Grasskamp, Alistair Hume, Bruno Goud, Bruno Baron, Patrick England, Margaret A Titus, Petra Schwille, Thomas Weidemann, Anne Houdusse, Eugen Kerkhoff
Institutions: Centre National de la Recherche Scientifique, Université Paris Sciences et Lettres, Biologie cellulaire et Cancer, Institut Curie, University Hospital Regensburg, Max Planck Institute of Biochemistry, Max Planck Institute for Biophysical Chemistry, University of Nottingham, Institut Pasteur, Biologie Moléculaire Structurale et Processus Infectieux, University of Minnesota
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Awards: G2028218037, G217303827, Paris Sciences et Lettres, G268148670, G2690507794, G3089088799, G3941723509, G4190806336, G4500396706, G471331628, G591834160, Early Chemotactic Signaling Mediated by an Unconventional Myosin, G628180677, G6358499467, G6730640092, G6759105742, G7240651115, G782245399, G7878337167, G7938061157, Cellular functions of Myosin V and Myosin X – Role of partners and specific design, G8475995907, G8733719073
OpenAlex ID: W2519817583