Work

Nucleosomal Barrier to Transcription: Structural Determinants and Changes in Chromatin Structure

Year: 2016

Type: article

Abstract: Packaging of DNA into chromatin affects all processes on DNA. Nucleosomes present a strong barrier to transcription, raising important questions about the nature and the mechanisms of overcoming the barrier. Recently it was shown that DNA sequence, DNA-histone interactions and backtracking by RNA polymerase II (Pol II) all contribute to formation of the barrier. After partial uncoiling of nucleosomal DNA from histone octamer by Pol II and backtracking of the enzyme, nucleosomal DNA recoils on the octamer, locking Pol II in the arrested state. Histone chaperones and transcription factors TFIIS, TFIIF and FACT facilitate transcription through chromatin using different molecular mechanisms.

Source: Biochemistry & Molecular Biology Journal

DOI: 10.21767/2471-8084.100017

Authors: Vasily M Studitsky, Ekaterina V Nizovtseva

Institutions: Fox Chase Cancer Center, Lomonosov Moscow State University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid