Work

Revealing Higher Order Protein Structure Using Mass Spectrometry

Year: 2016

Type: article

Abstract: The development of rapid, sensitive, and accurate mass spectrometric methods for measuring peptides, proteins, and even intact protein assemblies has made mass spectrometry (MS) an extraordinarily enabling tool for structural biology. Here, we provide a personal perspective of the increasingly useful role that mass spectrometric techniques are exerting during the elucidation of higher order protein structures. Areas covered in this brief perspective include MS as an enabling tool for the high resolution structural biologist, for compositional analysis of endogenous protein complexes, for stoichiometry determination, as well as for integrated approaches for the structural elucidation of protein complexes. We conclude with a vision for the future role of MS-based techniques in the development of a multi-scale molecular microscope. Graphical Abstract ᅟ.

Source: Journal of the American Society for Mass Spectrometry

DOI: 10.1007/s13361-016-1385-1

Authors: Brian T. Chait, Martine Cadene, Paul Dominic Olinares, Michael P. Rout, Yi Shi

Institutions: Rockefeller University, Centre National de la Recherche Scientifique, Centre de Biophysique Moléculaire

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources

Awards: Natl Resource for Mass Spectrom of Biolog Macromolecules, G2785835504, National Center for Dynamic Interactome Research, G5860052999, National Resource for Mass Spectrom of Biological Macromolecules