Year: 2005
Type: reference-entry
Abstract: In less than a year, SARS-CoV-2 managed to displace Mycobacterium tuberculosis (Mtb) as the leading cause of death worldwide due to a single infectious agent.Both pathogens affect the respiratory tract, mainly the lungs.However, the impact that a possible Mtb + SARS-CoV-2 co-infection can have on the host response is still unknown.Herein we depict a rigorous system to evaluate the complex interaction between two simultaneous infections in vitro in a lung epithelial cell line (A549).Overall, the process includes eight steps: (1) Mtb culture, (2) cell maintenance, (3) preparation of viral stocks, (4) determination of infectious titers, (5) Mtb and SARS-CoV-2 co-infection, (6) determination of intracellular bacterial load, (7) SARS-CoV-2 viability test, and (8) optional UV-C validation assay for infectivity reduction.This comprehensive protocol will allow experimentalists to study the pathogenesis of co-infection in vitro and facilitate collaborative work in the literature.
Source: John Wiley & Sons, Inc. eBooks
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G22113365, G5015586306, G7767678348
OpenAlex ID: W2520102457