Year: 2014
Type: article
Abstract: SUMOylation is a post-translational modification in which SUMO is covalently conjugated to a variety of proteins that results in the regulation of a range of cellular processes including cell proliferation and maintenance of genome stability.As described in the previous chapter, we found that SUMO-1 marks chromatin at the proximal promoter regions of some of the most active housekeeping genes during interphase.In this chapter, we show our work to identify SUMO substrates on the chromatin bound to these promoters and the effect on transcription regulation.We found that SUMO-1 marks the promoters via the Scaffold Associated Factor B (SAFB) protein.We found that depletion of SAFB disrupted the organization of Cajal body, which plays an important role for assembly and modification of the nuclear-transcription and RNA-processing machinery.This study found an unexpected role of SAFB that coupling transcription initiation and RNA processing process.
Author: Huiwen Liu
Institution: The Ohio State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2521117217