Work

Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus

Year: 1999

Type: article

Abstract: A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5-C down arrow TCGAG-3 as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined.

Source: NOT FOUND REPOSITORY (Indian Institute of Science Bangalore)

Authors: Siddamadappa Chandrashekaran, Aparna Bhavani Shankar, Padmanabhan Babu, Bindu D. Paul, Valakunja Nagaraja

Institution: Indian Institute of Science Bangalore

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2521754448

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