Work

Partition functions of unit-copy plasmids can stabilize the maintenance of plasmid pBR322 at low copy number

Year: 1986

Type: article

Abstract: The maintenance of plasmid pBR322 is highly unstable in a polA12 strain of Escherichia coli at 29 degrees C due to severely reduced copy number. Under these conditions, introduction of the par (partition) locus of plasmid P1 or the par (sop) region of F into pBR322 stabilizes it. A region with similar activity was detected in the P7 plasmid. The activity of the P1 par locus was dependent on the P1 parA gene product and was sensitive to par-specified incompatibility.

Source: Journal of Bacteriology

DOI: 10.1128/jb.168.2.1010-1013.1986

Authors: S Austin, S Friedman, D Ludtke

Institutions: Frederick National Laboratory for Cancer Research, National Cancer Institute

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Awards: G1129866861, RESEARCH AT THE NCI FREDERICK CANCER RESEARCH FACILITY