Work

Viral Immunofluorescence with Rift Valley Fever Virus Infected MEFs in a 96 Well Plate

Year: 2012

Type: article

Abstract: Immunofluorescence is a method to detect viral infection in multiple types of host cells. This procedure can be adapted for both high-throughput and low-throughput assays for any virus for which there are antibodies available. Time of infection and virus multiplicity of infection (MOI) vary and should be optimized for each virus and host cell type. Here we give an example of viral immunofluorescence in a 96 well plate with a Rift Valley fever virus (RVFV, strain MP12) infection in mouse embryonic fibroblasts (MEF).

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.297

Authors: Theresa Moser, Sara Cherry

Institution: University of Pennsylvania

Language: English

Topic: Viral Infections and Vectors

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Awards: G1586078693, G2223395877, Middle Atlantic Regional Center for Excellence for Biodefense and Emerging Infect