Year: 2012
Type: article
Abstract: ligands to stable non-reducible DNA NPs.The non-covalent biotin-streptavidin (SA) interaction was used for ligand conjugation after covalent methods proved inefficient.Biotinylated DNA NPs were prepared by compacting plasmid DNA with biotin-PEG-pLL block copolymer.A fusion protein of SA with the TAT peptide from the HIV-1 Tat protein was cloned, expressed, purified and attached to the biotinylated DNA NPs.A modified SA system with tetrameric structure but monovalent biotin binding capacity was adopted and shown to reduce the aggregation of biotinylated DNA NPs compared to NeutrAvidin.Compared to unmodified DNA NPs, TAT modified DNA NPs significantly enhanced in vitro gene transfer, particularly at low DNA concentrations.When injected intratracheally into wild-type mice, TAT modified DNA NPs showed efficient gene transfer at low doses of DNA, but the enhancement compared to unmodified DNA NPs was not statistically significant.Further in vivo study is necessary to establish the in vivo benefit of attaching TAT to DNA NPs.
Author: Wenchao Sun
Institution: Case Western Reserve University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2522072384