Full-length model of the human galectin-4 and insights into dynamics of inter-domain communication
Year: 2016
Type: article
Abstract: Galectins are proteins involved in diverse cellular contexts due to their capacity to decipher and respond to the information encoded by β-galactoside sugars. In particular, human galectin-4, normally expressed in the healthy gastrointestinal tract, displays differential expression in cancerous tissues and is considered a potential drug target for liver and lung cancer. Galectin-4 is a tandem-repeat galectin characterized by two carbohydrate recognition domains connected by a linker-peptide. Despite their relevance to cell function and pathogenesis, structural characterization of full-length tandem-repeat galectins has remained elusive. Here, we investigate galectin-4 using X-ray crystallography, small- and wide-angle X-ray scattering, molecular modelling, molecular dynamics simulations, and differential scanning fluorimetry assays and describe for the first time a structural model for human galectin-4. Our results provide insight into the structural role of the linker-peptide and shed light on the dynamic characteristics of the mechanism of carbohydrate recognition among tandem-repeat galectins.
Source: Scientific Reports
DOI: 10.1038/srep33633
Institutions: Universidade de São Paulo, Princeton University
Language: English
Topic: Galectins and Cancer Biology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: National Science Foundation, U.S. Department of Energy, Rice University, Diamond Light Source, Fundação de Amparo à Pesquisa do Estado de São Paulo, Universidade de São Paulo, National Institutes of Health, Office of Science, National Institute of General Medical Sciences, Division of Materials Research, Basic Energy Sciences, SLAC National Accelerator Laboratory
Awards: G1229526393, G1286251239, Structural Characterizations of Transient and Heterogeneous Protein Complexes, G1920558564, G21810547, G2342063303, Structural characterization and evaluation of funciontal aspects of tanden-repeat human galectins, G2371548206, G2579886042, G262532246, G303061748, G4102202665, G4121408022, G4235786860, G440393388, G4581610328, G4611182784, G4654690619, G495301720, G498139845, G5087724830, G522049510, OPERATION OF CORNELL HIGH ENERGY SYNCHROTRON SOURCE (CHESS), G5537587939, G5570207115, G5724513577, MRI-R2: Acquisition of Data Analysis and Visualization Cyber-Infrastructure for Computational Science and Engineering Applications(DAVinCI), G6093986502, G6123939489, G6341728005, G636769351, G6429492609, G6550344761, Structural Characterizations of Transient and Heterogeneous Protein Complexes, G6971479070, G7283024673, G8382000481, Macromolecular Diffraction Resource: MacCHESS, G8925327636, G9000264529, G9000339200, G911061179, G969889393
OpenAlex ID: W2522276088