Encapsulation of in Vitro Proliferated Buds of Olive
Year: 1998
Type: article
Abstract: Encapsulation in nutrient-enriched alginate capsules of buds excised from in vitro proliferated shoots can be considered an important contribution to micropropagation, for germplasm preservation and/or for the synthetic seed technology. Four experiments were carried out to evaluate the aptitude of nodal and apical buds of olive ( Olea europea L., cv. Moraiolo) toward encapsulation in sodium alginate and to study the effect of some root induction treatments and of growth regulators in the capsule or in the sowing medium. In all cases, after 45 days from sowing in agar medium, the encapsulated apical buds showed higher viability and regrowth ability (sprouting rate, shoot length and basal callus formation) than the nodal ones. Moreover, the encapsulated buds can be conveniently stored at 4°C, but not for longer than 45 days; a different behaviour between apical and nodal microcuttings was monitored also after storage. Root induction treatments before encapsulation of the buds resulted in low percentages of plantlet regeneration, which was, in any case, obtained only from apical microcuttings. Modifying the indol-butyric acid concentration in the rooting induction phase and/or the composition of the nutrient-enriched capsule and/or the sowing medium usually increased plantlet regeneration rates up to a maximum of 30%.
Authors: Maurizio Micheli, Maurizio Mencuccini, A. Standardi
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2523872767