Work

Characterization of a new Gsx2‐cre line in the developing mouse telencephalon

Year: 2016

Type: article

Abstract: In this study, we generated a transgenic mouse line driving Cre and EGFP expression with two putative cis‐regulatory modules (CRMs) (i.e., hs687 and hs678) upstream of the homeobox gene Gsx2 (formerly Gsh2), a critical gene for establishing lateral ganglionic eminence (LGE) identity. The combination of these two CRMs drives transgene expression within the endogenous Gsx2 expression domains along the anterior–posterior neuraxis. By crossing this transgenic line with the RosatdTomato (Ai14) reporter mouse line, we observed a unique recombination pattern in the lateral ventral telencephalon, namely the LGE and the dorsal half of the medial GE (MGE), but not in the septum. We found robust recombination in many cell types derived from these embryonic regions, including olfactory bulb and amygdala interneurons and striatal projection neurons from the LGE, as well as cortical interneurons from the MGE and caudal GE (CGE). In summary, this transgenic mouse line represents a new tool for genetic manipulation in the LGE/CGE and the dorsal half of MGE.

Source: genesis

DOI: 10.1002/dvg.22980

Authors: Shenyue Qin, Mayur Madhavan, Ronald R. Waclaw, Masato Nakafuku, Kenneth Campbell

Institutions: Cincinnati Children's Hospital Medical Center, University of Cincinnati

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: Molecular control of neurogenesis in the adult subventricular zone, The Roles of Gsh1 and Gsh2 Genes in Telencephalic Neurogenesis, Roles of Gsx factors in telencephalic neurogenesis