Work

Fluorescence-guided surgery of a highly-metastatic variant of human triple-negative breast cancer targeted with a cancer-specific GFP adenovirus prevents recurrence

Year: 2016

Type: article

Abstract: // Shuya Yano 1, 2, 3 , Kiyoto Takehara 1, 2, 3 , Shinji Miwa 1, 2 , Hiroyuki Kishimoto 3 , Hiroshi Tazawa 4 , Yasuo Urata 5 , Shunsuke Kagawa 3 , Michael Bouvet 2 , Toshiyoshi Fujiwara 3 , Robert M. Hoffman 1, 2 1 AntiCancer, Inc., San Diego, CA, USA 2 Department of Surgery, University of California San Diego, CA, USA 3 Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan 4 Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan 5 Oncolys BioPharm Inc., Tokyo, Japan Correspondence to: Robert M. Hoffman, email: [email protected] Toshiyoshi Fujiwara, email: toshi_ [email protected] Keywords: fluorescence-guided surgery (FGS), telomerase dependent, adenovirus, GFP/RFP, survival Received: July 07, 2016 Accepted: September 06, 2016 Published: September 28, 2016 ABSTRACT We have previously developed a genetically-engineered GFP-expressing telomerase-dependent adenovirus, OBP-401, which can selectively illuminate cancer cells. In the present report, we demonstrate that targeting a triple-negative high-invasive human breast cancer, orthotopically-growing in nude mice, with OBP-401 enables curative fluorescence-guided surgery (FGS). OBP-401 enabled complete resection and prevented local recurrence and greatly inhibited lymph-node metastasis due to the ability of the virus to selectively label and subsequently kill cancer cells. In contrast, residual breast cancer cells become more aggressive after bright (white)-light surgery (BLS). OBP-401-based FGS also improved the overall survival compared with conventional BLS. Thus, metastasis from a highly-aggressive triple-negative breast cancer can be prevented by FGS in a clinically-relevant mouse model.

Source: Oncotarget

DOI: 10.18632/oncotarget.12314