Mitochondrial Network Dynamics in Vascular Endothelial Cells Exposed to Mechanochemical Stimuli: Experimental and Mathematical Analysis
Year: 2012
Type: article
Abstract: This work was conducted in order to assess the role that the mitochondrial network plays in cardiovascular disease.More specifically, we first conducted studies to determine which bioenergetic factors control changes in the mitochondrial network by exposing cultured endothelial cells to specific chemical treatments.Following this work, we used a parallel plate flow chamber and media recirculation system to expose cultured endothelial cells to simulated ischemia (I)/ reperfusion (RP) injury in order to determine if changes in the mitochondrial network occur upon I/RP and delineate the molecular mechanisms that govern them.The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state.The equilibrium between mitochondrial fusion/fission, as well as the organelle motility, determine network morphology and ultimately mitochondrial/cell function.Network morphology has been linked with the energy state in different cell types.As stated, in the first part of this study, we examined how bioenergetic factors affect mitochondrial dynamics/motility in cultured vascular endothelial cells (ECs).ECs were transduced with mitochondria-targeted green fluorescent protein (mito-GFP) and exposed to inhibitors of oxidative phosphorylation (OXPHOS) or ATP synthesis.Time-lapse fluorescence videos were acquired and a
Author: Randy J. Giedt
Institution: The Ohio State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2527709109