Year: 2012
Type: dissertation
Abstract: With the objective of replacing the need of living animals to test chemicals respectively their reactions on the eye we created the EVEIT System. Therefore we designed a perfusion chamber in which rabbit corneas of animals that were killed for the food industry were clamped. Their nutrition was guaranteed by a special nutrition medium and the whole setup was stored in a breeder which ensured a constant temperature of 31°C. We did testings in mechanical and chemical ways on these cultivated corneas besides untreated control groups. The corneas were evaluated by morphological (endothelial and epithelial examinations) and biochemical (pH, glucose-lactate-metabolism, cytokines, growth factors in the medium) criteria. We could show that in principle a total healing of the cornea and a recover of the glucose-lactate-metabolism is possible after trauma. Different chemicals presented varying patterns of trauma and wound healing. The testings with different concentrations of the chemicals give at least a hint of dose response. But further testings with bigger groups of treated corneas and other concentrations need to be done to give better statements.
Source: RWTH Publications (RWTH Aachen)
Authors: Miriam Goß, Norbert Schrage
Language: German
Subfield: Small Animals
Field: Veterinary
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2528433831