Year: 2014
Type: conference-paper
Abstract: In this work, the fabrication and testing of a miniaturized device to perform all steps necessary for digital droplet polymerase chain reaction (ddPCR) within a single chip is presented. The microfluidic chip, made in silicon, contains a T-junction for droplet generation along with a microreactor for temperature cycling. The temperature is controlled by means of an external, miniaturized thermoelectric element. The number of fluorescent droplets after PCR amplification is determined using a fluorescent microscope; furthermore, individual droplet fluorescence is recorded at each cycle, thus enabling real time PCR analysis. The microreactor accommodates about 2500-3000 droplets of uniform volume varying in the 55-65 pL range, allowing the direct and accurate detection of human genomic DNA in concentrations from 0.06 to 40 ng/μL.
Source: Lirias
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2528976671