Toward Noninvasive Diagnosis of IgA Nephropathy: A Pilot Urinary Metabolomic and Proteomic Study
Year: 2016
Type: article
Study design: Observational Study
Abstract: IgA nephropathy is diagnosed by renal biopsy, an invasive procedure with a risk of significant complications. Noninvasive approaches are needed for possible diagnostic purposes and especially for monitoring disease activity or responses to treatment. In this pilot project, we assessed the utility of urine samples as source of biomarkers of IgA nephropathy. We used spot urine specimens from 19 healthy controls, 11 patients with IgA nephropathy, and 8 renal-disease controls collected on day of renal biopsy. Urine samples were analyzed using untargeted metabolomic and targeted proteomic analyses by several experimental techniques: liquid chromatography coupled with mass spectrometry, immunomagnetic isolation of target proteins coupled with quantitation by mass spectrometry, and protein arrays. No single individual biomarker completely differentiated the three groups. Therefore, we tested the utility of several markers combined in a panel. Discriminant analysis revealed that combination of seven markers, three metabolites (dodecanal, 8-hydroxyguanosine, and leukotriene C4), three proteins (α1-antitrypsin, IgA-uromodulin complex, and galactose-deficient IgA1), and heparan sulfate, differentiated patients with IgA nephropathy from patients with other renal diseases and healthy controls. Future studies are needed to validate these preliminary findings and to determine the power of these urinary markers for assessment of responses to therapy.
Source: Disease Markers
DOI: 10.1155/2016/3650909
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,550
Awards: Molecular Basis of Pathogenicity of IgA1-containing Immune Complexes, G2129936067, G2380713203, Elucidating IgA nephropathy through Genetic Studies of IgA1 Glycosylation, G3041298778, Prague University Analytical Centre II and III - NPU 2015-2020, G4277618395, G481350182, G4885064193, G4928754176, G5135245572, Prague University Analytical Center for Health, Food Safety and Environmental Protection, G5311849225, G6693508342, UAB-UCSD O'Brien Center for Acute Kidney Injury Research, G7023612179, G7404817890, Abnormal STAT3 signaling and aberrant O-glycosylation of IgA1 in IgA nephropathy, G7970576604, G8187705307, G8566688478, G8830341093, Future materials, G893273624, G9000019921, G9000064520, G9000325384, Molecular Basis of Pathogenicity of IgA1-containing Immune Complexes, G998136849
OpenAlex ID: W2529130100