Characterization of Stimulation-induced Volume Changes in the Ca1Region of Rat Hippocampus Slices
Year: 2013
Type: article
Abstract: Characterization of stimulation-induced volume changes of the CA1 region within in vitro rat hippocampus slices.Intrinsic optical signals (IOS) were used to evaluate volume regulation mechanisms during Schaffer collateral stimulation-induced volume changes in hippocampal slices.The effects of stimulation frequency, synaptic function, ionotropic glutamate receptor (iGluR) activation, glutamate uptake, and volume regulatory anion channels (VRAC) were examined with IOS.Data were analyzed using ANOVA with Dunnett's post hoc test (p<0.05indicated significance).IOS changes were stimulation frequency dependent between 1 Hz and 10 Hz with full recovery of IOS within 5 min.Synaptic blockage reduced the rate of swelling by 81% compared to the control and the IOS did not fully recover.Recovery rate was reduced with iGluR inhibition.Inhibition of glutamate uptake reduced the rate of swelling by 70% compared to vehicle controls.During stimulation, action potentials, iGluR activation, and glutamate uptake contribute to swelling.Regulatory volume mechanisms during stimulation were glutamate-mediated via iGluRs and VRAC independent.
Author: Amanda Brooke Gutwein
Institution: Wright State University
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2529986851